Overview
This protocol reproducibly generates competent cultures of E. coli that yield 1 x 108 to 3 x 108 transformed colonies/µg of plasmid DNA. The protocol works optimally when the bacterial culture is grown at 18°C. If a suitable incubator is not available, a standard bacterial shaker can be set up in a 4°C cold room and regulated to 18°C. By Joseph Sambrook and David W. Russell.
Details
Details of this protocol, The Inoue Method For Preparation And Transformation Of Competent E. Coli:, are located on a web site other than Biocompare Protocols.