Preparation And Transformation Of Competent E. Coli Using Calcium Chloride - Subscription Required Protocol

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Title	Preparation And Transformation Of Competent E. Coli Using Calcium Chloride - Subscription Required
Source	CSH Protocols; 2006; doi:10.1101/pdb.prot3932

Overview

This protocol, developed approx. 30 years ago, is used to prepare batches of competent bacteria that yield 5 x 106 to 2 x 107 transformed colonies/µg of supercoiled plasmid DNA. By Joseph Sambrook and David W. Russell.

Details

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