Fixing Suspension Cells With Paraformaldehyde-SUBSCRIPTION REQUIRED Protocol

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Protocol Information

Title Fixing Suspension Cells With Paraformaldehyde-SUBSCRIPTION REQUIRED
Source CSH Protocols; 2006; doi:10.1101/pdb.prot4295

Overview

Treating cells with paraformaldehyde leads to the establishment of chemical cross-links between free amino groups. When the cross-links join different molecules, a latticework of interactions occurs that holds the overall architecture of the cell together. Commercial formaldehyde solutions are not recommended, because they lack the advantages of using a variable-length polymer.
Ed Harlow and David Lane

Details

Details of this protocol, Fixing Suspension Cells With Paraformaldehyde-SUBSCRIPTION REQUIRED, are located on a web site other than Biocompare Protocols.

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