Overview
A simple method for handling suspension cells is to attach them to a solid substrate by chemical linkage before fixation. For chemical linkage, a positively charged polymer (commonly poly-L-lysine or a commercial equivalent such as Vectabond) provides a site for binding of cells, which carry an overall negative charge.
Ed Harlow and David Lane
Details
Details of this protocol, Attaching Suspension Cells To Slides Using Chemical Linkers-SUBSCRIPTION REQUIRED, are located on a web site other than Biocompare Protocols.