Overview
This protocol describes a method for constant-flow microinjection using the Pneumatic PicoPump (World Precision Instruments). This type of system is very simple and can be assembled on a relatively low budget. In this method, a constant flow of sample is delivered from the tip of the pipette, and the amount of sample injected into the cell is determined by how long the pipette remains in the cell. By David A. Dean
Details
Details of this protocol, Gene Delivery By Direct Injection (Microinjection) Using A Controlled-Flow System - Subscription Required, are located on a web site other than Biocompare Protocols.