Overview
Fluorochrome-linked reagents should be used when high resolution is needed or if two antigens need to be localized simultaneously. Because of the thickness of fly specimens, detection requires access to a confocal microscope.
Ed Harlow and David Lane
Details
Details of this protocol, Antibody Addition To Drosophila Specimens And Detection Using Fluorochrome-Linked Reagents (Subscription Required), are located on a web site other than Biocompare Protocols.