Overview
Where possible, it is preferable to use denaturing IPs to recover labeled proteins from pulse-chase experiments. The use of denaturing IPs reduces background and simplifies protein harvest. Yeast are boiled in an SDS-containing solution to liberate total proteins. This is also much safer than bead-beating radioactive yeast samples.
William P. Tansey
Details
Details of this protocol, Denaturing Protein Immunoprecipitation From Yeast (Subscription Required), are located on a web site other than Biocompare Protocols.