Overview
This protocol describes a method for producing ES cell-tetraploid embryo chimeras. It requires the timed combination of four-cell-stage tetraploid embryo production and the procedure for ES cell-diploid embryo aggregation in which diploid embryos are replaced with tetraploid embryos. The resulting chimeras can be used to analyze the embryonic versus extraembryonic phenotype of a mutation. By Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer.
Details
Details of this protocol, Assembling Aggregates Between Embryonic Stem (ES) Cells And Tetraploid Embryos - Subscription Required, are located on a web site other than Biocompare Protocols.