Overview
This protocol describes a method for producing diploid embryo-tetraploid embryo chimeras. It requires the timed combination of four-cell-stage tetraploid embryo production and the procedure for diploid embryo-diploid embryo aggregation. The resulting chimeras are useful for phenotypic analysis when an induced mutation has an extraembryonic phenotype. By Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer.
Details
Details of this protocol, Assembling Aggregates Between Diploid And Tetraploid Embryos - Subscription Required, are located on a web site other than Biocompare Protocols.