Overview
This method allows precise analysis of methylation in a certain region by converting all nonmethylated cytosines into tymines, while methylated cytosines remain unchanged. This method requires small amount of genomic DNA and therefore seems to be very useful for the analysis of clinical samples, where the material amount is limited. However I suggest optimising the method using genomic DNA from a cell line and then apply it to valuable samples. Contributed by Dr.A.Gratchev, University of Heidelberg, Germany.
Details
Details of this protocol, Analysis Of DNA Methylation Using Bisulphite Sequencing, are located on a web site other than Biocompare Protocols.