Overview
The following protocol describes the preparation of an shRNA-expressing Gateway entry vector. This process is preceded by the design and synthesis of target gene-specific sense and antisense oligonucleotides which, when annealed, will constitute an shRNA cassette.
Details
Details of this protocol, Annealing, Cloning, And Sequencing Of ShRNA Linkers Into PEN_H1 Plasmids, are located on a web site other than Biocompare Protocols.