Overview
The method chosen for measuring the content of cyclic adenosine 3',5'-
monophosphate (cyclic AMP or cAMP) in RAW 264.7 cells is an enzyme-linked
immunoassay system developed by Amersham Biosciences. An overview of the
assay, cAMP Biotrak EIA, and its instructions, Biotrak Protocol, are available
through the indicated links. The procedure described below provides a sample
sufficient for one determination of cAMP using the acetylation protocol
Details
Details of this protocol, Preparation Of RAW 264.7 Lysates For Cyclic AMP Determination, are located on a web site other than Biocompare Protocols.