Overview
The method chosen for measuring the content of cyclic adenosine 3',5'-
monophosphate (cyclic AMP or cAMP) in splenic B lymphocytes (B cells) is an
enzyme-linked immunoassay developed by Amersham Biosciences. An overview
of the assay, cAMP Biotrak EIA, and its instructions, Biotrak Protocol, are
available through the indicated links. The procedure described below follows the
basic instructions of the Biotrak System, Protocol 2, for the analysis of total
cAMP in cultured cells (includes extracellular and intracellular cAMP). This
protocol utilizes acetylation of cAMP to improve sensitivity and reduce
interference. Lysates of cells are made and stored at -80 C.
Details
Details of this protocol, Assay Of Cyclic AMP In Lysates Of Cells, are located on a web site other than Biocompare Protocols.