Visualization Of F-Actin And G-Actin Equilibrium Using FRET In Cultured Cells And Neurons In Slices (Subscription Required) Protocol

Protocol Search

Please enter a search word

Protocol Information


Title	Visualization Of F-Actin And G-Actin Equilibrium Using FRET In Cultured Cells And Neurons In Slices (Subscription Required)
Source	Nature Protocols: Okamoto et al., RIKEN-MIT Neuroscience Research Center, MIT

Overview

Here we describe an optical approach that allows us to monitor F-actin and G-actin equilibrium in living cells through the use of two-photon microscopy to observe fluorescence resonance energy transfer (FRET) between actin monomers.

Details

Details of this protocol, Visualization Of F-Actin And G-Actin Equilibrium Using FRET In Cultured Cells And Neurons In Slices (Subscription Required), are located on a web site other than Biocompare Protocols.