Single- and Dual-Parameter FRET Kinase Probes Based On Pleckstrin Protocol

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Title	Single- and Dual-Parameter FRET Kinase Probes Based On Pleckstrin
Source	Nature Protocols: Brumbaugh et al., European Molecular Biology Institute, Heidelberg, Germany.

Overview

Here we describe protocols for preparing and using fluorescent probes that respond to conformational changes by altered Foerster resonance energy transfer (FRET) efficiencies upon phosphorylation or, in principle, other posttranslational modifications (PTMs).

Details

Details of this protocol, Single- and Dual-Parameter FRET Kinase Probes Based On Pleckstrin, are located on a web site other than Biocompare Protocols.