Si-RNA Transfection Of Mouse Bone Marrow Progenitors By Electroporation Protocol

Protocol Search

Protocol Information

Title Si-RNA Transfection Of Mouse Bone Marrow Progenitors By Electroporation
Source Daniela Oliveira and Margaret Goodell, Baylor College of Medicine.

Overview

This protocol was devised for electroporation of mouse bone marrow cells that have been enriched for progenitors by magnetic enrichment for the Sca-1 antigen (using the Miltenyi system). We initially determined optimal electroporation conditions for siRNA by transfecting fluorescently-labeled siRNA oligonucleotides obtained from Orbigen.

Details

Details of this protocol, Si-RNA Transfection Of Mouse Bone Marrow Progenitors By Electroporation, are located on a web site other than Biocompare Protocols.

read more...
advertisement

Email Newsletter Sign-Up

Stay updated on the latest technologies and news with Biocompare's newsletters
(See samples here)









Select All

Loading

Loading