Protocol For The Isolation Of A Heterogenous Muscle-Derived Cell Population

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Title Protocol For The Isolation Of A Heterogenous Muscle-Derived Cell Population
Source S. L.McKinney, K. J. Jackson, T. Mi, S. Majka, Margaret A. Goodell, Baylor College of Medicine.

Overview

This protocol is employed for the purification of a population of muscle-derived cells from skeletal muscle of C57Bl/6 animals. The resulting preparation is a mixture of many cell types including satellite cells (a.k.a., muscle progenitor cells) and hematopoietically active muscle-derived cells. This protocol is based on that described by Yablonka- Reuveni et al., with slight modifications1.

Details

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