Overview
The above protocol is a pulse-chase procedure designed to label only the vacuole
membranes of yeast cells with FM 4-64. FM 4-64 is a vital stain: it fluoresces only in living cells, so cells cannot be fixed then stained nor stained then fixed. You must stain and observe living cells.
Details
Details of this protocol, FM 4-64 Labeling Of Yeast Vacuole Membranes, are located on a web site other than Biocompare Protocols.