Overview
We describe here a high sensitivity indirect detection procedure for DIG-labeled hybridization probes. The procedure uses the components of the HNPP Fluorescent Detection
Set to form a fluorescent precipitate of HNPP (2-hydroxy-3-naphthoic acid-2’-phenylanilide
phosphate) and Fast Red TR at the site of hybridization. This procedure can be used to detect single copy sequences as small as 1 kb on human metaphase chromosomes.
Details
Details of this protocol, DNA In Situ Hybridization With An Alkaline Phosphatase-Based Fluorescent Detection System, are located on a web site other than Biocompare Protocols.