A Simplified & Efficient Protocol For ISH To Polytene Chromosomes With A DIG-Labeled DNA Probe

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Protocol Information

Title A Simplified & Efficient Protocol For ISH To Polytene Chromosomes With A DIG-Labeled DNA Probe
Source Roche Applied Science: Non-radioactive In Situ Hybridization Application Manual

Overview

The protocol given here is a derivative of several published methods (Langer-Safer et al., 1982; Schmidt et al., 1988) with some minor modifications that make the method of in situ hybridization easier, faster, more reliable, and available to anyone who can operate a microscope.

Details

Details of this protocol, A Simplified & Efficient Protocol For ISH To Polytene Chromosomes With A DIG-Labeled DNA Probe, are located on a web site other than Biocompare Protocols.

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