Overview
The procedure described here incorporates one modified nucleotide (DIG-,biotin-, fluorescein-, AMCA-, or tetramethylrhodamine-dUTP) at approximately every 20 – 25th position in the newly synthesized DNA. This labeling density allows optimal enzymatic incorporation of the modified nucleotide and produces the most sensitive targets for indirect (immunological) detection.
Details
Details of this protocol, Nick-Translation Labeling Of Ds DNA With Nick Translation Mixes For In Situ Probes, are located on a web site other than Biocompare Protocols.