Flow Cytometry After Bromodeoxyuridine Labeling To Measure S And G2+M Phase Durations Protocol

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Protocol Information

Title Flow Cytometry After Bromodeoxyuridine Labeling To Measure S And G2+M Phase Durations
Source Nicholas HA Terry, Department of Experimental Radiation Oncology, University of Texas.

Overview

This protocol describes methods for calculating the proliferative parameters of cell populations. The basis of the technique is to label cells, either in vitro or in vivo, with halogenated thymidine analogs, such as bromodeoxyuridine (BrdU). Bivariate DNA-BrdU flow cytometry is used to analyze the BrdU-labeled and unlabeled cells.

Details

Details of this protocol, Flow Cytometry After Bromodeoxyuridine Labeling To Measure S And G2+M Phase Durations, are located on a web site other than Biocompare Protocols.

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