Isolation Of Arabidopsis CDNAs By Complementation In Yeast Protocol

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Protocol Information

Title Isolation Of Arabidopsis CDNAs By Complementation In Yeast
Source Dr. Brian Keith, University of Chicago & Dr. Judith Bender, Johns Hopkins University.

Overview

An effective strategy for isolating Arabidopsis homologs of other eukaryotic genes is to screen or select for Arabidopsis cDNAs in a yeast expression vector that can complement the appropriate yeast mutant. Protocol from the Cold Spring Harbor Arabidopsis Molecular Genetics Course July 1996.

Details

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