Overview
To identify the YAC subclones containing both a human insert and a portion of either the left or right arm of the pYAC4 vector. Identification of these clones is necessary in order to do YAC chromosome walking, and is also useful in the determination of whether a particular YAC clone has a contiguous human insert or whether a co-cloning event has occurred. Vector arm sequences are identified using pBR322 fragments from a BamHI-PvuII double digest. Note: A PCR procedure for finding endclones is now used in the lab. See J. Howe for the protocol.
Details
Details of this protocol, Identification Of End Clones In YAC Subclone Libraries, are located on a web site other than Biocompare Protocols.