Subcloning Of Yeast Artificial Chromosomes Into Phage Lambda Protocol

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Protocol Information

Title Subcloning Of Yeast Artificial Chromosomes Into Phage Lambda
Source Donis Keller lab manual - Washington University in St. Louis

Overview

To subclone the large insert fragment of human DNA contained within a YAC into a bacteriophage lambda vector. The subclones are 15 to 23 kb in size, and can be used to identify new polymorphic markers from a known region of the genome, to map a specific locus, and/or to screen other libraries.

Details

Details of this protocol, Subcloning Of Yeast Artificial Chromosomes Into Phage Lambda, are located on a web site other than Biocompare Protocols.

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